3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1) Antibody

Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98% identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.

Target	3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB
Host	Rabbit
Recommended dilutions	WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 592-624 amino acids from the C-terminal region of human PAPSS1.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	O43252 (UniProt, ExPASy)
UniProt Secondary AC	O43841, O75332, Q6IAX6, Q96FB1, Q96TF4, Q9P1P9, Q9UE98
UniProt Entry Name	PAPS1_HUMAN
Gene Symbol	PAPSS1
NCBI Accession	NP_005434.4
KEGG	hsa:9061
String	9606.ENSP00000265174
Molecular Weight	Calculated MW: 70.8 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on 3'-Phosphoadenosine 5'-Phosphosulfate Synthase 1 (PAPSS1)

Write a review

Your Name

Your Email Address

PMID

DOI

Your Review

Rating

Continue