Coomassie Brilliant Blue Fast Staining Solution

Based on Coomassie G250 dye, Coomassie Brilliant Blue Fast Staining Solution can be used for fast and sensitive protein staining in SDS-PAGE or native PAGE, or used for detecting the remaining proteins on PAGE after Western transfer membrane. Instead of common toxic methanol and irritant acetic acid, this solution contains a non-toxic and non-irritant formulation. This solution can detect bands at 100 ng in electrophoresis gel within 1 hour, however, routine methods may need more than 3 hours.

Tested Applications	WB
Buffer	Contains Coomassie Brilliant Blue. The exact formulation is proprietary.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	After electrophoresis, wash the gel with distilled water by agitating 3 times, 5 min each time. Remove the distilled water, then immerse the gel in the minimum volume of Coomassie Brilliant Blue Fast Staining Solution. Agitate prior to use. Bands corresponding to large proteins will normally appear within 10-30 min; the majority of bands should appear within 1-2 hours. To obtain more sensitive bands or to speed up the staining, the gel can be immersed in Coomassie Brilliant Blue Fast Staining Solution and then heated in water bath or microwave oven set to 95-100 °C, followed by staining on a shaker. Cool the gel to room temperature, then repeat the heating-cooling steps 2-3 times. Avoid boiling the solution as this may cause the gel to break. The staining should be stopped when the target bands become apparent. Remove the staining solution, then add distilled water to stop the staining reaction. Photograph and record the results. Note: To obtain clearer bands, wash the gel with distilled water at room temperature, or immerse in distilled water at 4 °C overnight. Remove the distilled water and stain and wash the stained gel again (steps 2-4). Troubleshooting: No bands appear. The loading volume may be too low, it is recommended to set two BSA lanes with different volumes as positive controls during electrophoresis. High background. Interfering substances (such as salts, SDS) may not have been completely removed during the wash step. Increase the number of wash steps after electrophoresis. Precipitates appear when staining the gel. The container may be contaminated. It is recommended to change to a clean container, then add the fresh staining solution to continue staining until bands become apparent.

Research Articles on Coomassie Brilliant Blue Fast Staining Solution

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