Gel Stain

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Catalogue No: abx098146
Price: US$362.50
(Size: 500 µl)
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Gel Stain

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Datasheet
Gel Stain is a sensitive, stable and safe staining reagent for DNA/RNA. Gel Stain contains unique lipohilic macromolecules, which are incapable of entering cells via the cell membrane. Gel Stain is a suitable alternative for the highly toxic ethidium bromide (EtBr) for staining different sizes of dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. It can be heated or microwaved, and exhibits strong fluorescent signal from samples, with low background signal. It can be used before or after running the electrophoresis gel. No destaining is needed. Standard ethidium bromide (EB) filter and SYBR filter can be used. The optimal excitation is obtained with UV wavelength approximately at 300 nm.

This product is provided as a 10,000X concentrated solution.

Target Gel Stain
Storage Store at room temperature.
Availability Shipped within 10-20 working days.
Note This product is for research use only.
Directions for use Precast Staining Protocol:

Note: the pre-cast protocol is not recommended for polyacrylamide gels. Polyacrylamide gels can be stained using the post-staining protocol.

  1. Prepare agarose gel solution using standard protocols.
  2. For each 10 ml agarose gel solution, add 1 µl of Gel Stain 10,000X. Gel Stain can be added whilst the gel solution is still hot. Mix thoroughly.
  3. Cast the gel and allow it to solidify.
  4. Load samples and run the gels using standard protocols.
Post-Staining Protocol:
  1. Run gels according to standard protocols.
  2. Dilute Gel Stain 10,000X to 3X in a 0.1 M NaCl solution (e.g. add 15 µl of Gel Stain 10,000X to 5 ml of 1 M NaCl and 45 ml of distilled water).
  3. Place the gel in a suitable container. Add a sufficient quantity of 3X staining solution so that the gel is submerged.
  4. Place the mixture on an orbital shaker and gently mix for approximately 30 minutes. The optimal staining time will vary depending on gel thickness and % agarose. Most agarose gels require between 30 minutes to 1 hour.
  5. Destaining is not required, though the gel can be washed with distilled water to reduce background if necessary.
  6. The staining solution can be reused 2-3 times. Store at room temperature in the dark.
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