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AMPK alpha (pT172) Antibody

Catalogue No: abx000170
Price: £120.00
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Western blot analysis of extracts of C2C12 cells,using Phospho-AMPKα-T172 antibody (abx000170) at 1/2000 dilution. C2C12 cells were treated by Oligomycin (0.5uM) for 30 minutes, treated by serum-starvation overnight, treated by Hydrogen Peroxide (2nM) for 15 minutes or treated by AICAR (0.5mM) for 30 minutes after serum-starvation overnight.

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AMPKα (pT172) Antibody is a Rabbit Polyclonal antibody against AMPKα (pT172). The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Target AMPK alpha (pT172)
Clonality Polyclonal
Reactivity Mouse
Tested Applications WB
Host Rabbit
Recommended dilutions WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding S172 of human AMPKα.
Isotype IgG
Form Liquid
Purification Affinity purified.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q13131 (UniProt, ExPASy) P54646 (UniProt, ExPASy)
UniProt Secondary AC A8MTQ6, B2R7E1, O00286, Q5D0E1, Q86VS1, Q9UNQ4
UniProt Entry Name AAPK1_HUMAN
Gene Symbol PRKAA1, PRKAA2
GeneID 5562
String 9606.ENSP00000346148
Molecular Weight Calculated MW: 64 kDa, 65 kDa
Observed MW: 64 kDa
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
Concentration > 1 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on AMPK alpha (pT172) Antibody

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