Green Fluorescent Protein (GFP) Antibody

Green Fluorescent Protein (GFP) Antibody is a Mouse Monoclonal antibody against GFP. GFP, originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. This GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T.

Target	Green Fluorescent Protein (GFP)
Clonality	Monoclonal
Reactivity	Human
Tested Applications	ELISA, WB, IF/ICC
Host	Mouse
Recommended dilutions	WB: 1/20000, IF/ICC: 1/25. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	Purified His-tagged GFP protein
Isotype	IgG1
Form	Liquid
Purification	Purified by protein G and Ni-NTA nickel affinity chromatography, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Molecular Weight	Calculated MW: 27 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Green Fluorescent Protein (GFP)

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