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Intercellular Adhesion Molecule 1 (ICAM1) Antibody (PE)

Catalogue No: abx134368
Price: £250.00
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Intercellular Adhesion Molecule 1 (ICAM1) Antibody (PE) Intercellular Adhesion Molecule 1 (ICAM1) Antibody (PE)

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Mouse monoclonal antibody to CD54 (PE).

Target Intercellular Adhesion Molecule 1 (ICAM1)
Clonality Monoclonal
Reactivity Human
Tested Applications IF/ICC, FCM
Host Mouse
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Conjugation PE
Excitation/Emission 565/575
Laser Line 550
Isotype IgG1
Form Liquid
Purification Purified by affinity chromatography.
Storage Store at 4 °C. Do not freeze.
UniProt Primary AC P05362 (UniProt, ExPASy)
UniProt Secondary AC B2R6M3, Q5NKV7, Q96B50
UniProt Entry Name ICAM1_HUMAN
Gene Symbol ICAM1
GeneID 3383
KEGG hsa:3383
String 9606.ENSP00000264832
Buffer PBS, pH 7.3, 0.2% BSA, and 0.02% sodium azide.
Specificity Recognises human CD54.
Directions for use
  1. Take 100 µl peripheral blood anticoagulated by EDTA and add to the bottom of a 5 ml tube.
  2. Add 10 µl labeled antibody to the bottom of the flow tube and mix with the whole blood. Allow to stand for 20 minutes at room temperature in the dark.
  3. Add 2 ml RBC lysis buffer, mix, then incubate for 10 minutes in the dark to dissolve red blood cells.
  4. Centrifuge the sample tube at 1000 RPM for 5 minutes. Discard the supernatant.
  5. Add 2 ml PBS to resuspend and wash the cells, then centrifuge at 1000 RPM centrifugation for 5 minutes. Discard the supernatant.
  6. Add 0.5 ml PBS to resuspend the cells, and detect by flow cytometry (sample should be measured on the same day, or can be fixed overnight at 4 °C then measured).
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on Intercellular Adhesion Molecule 1 (ICAM1) Antibody (PE)

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