AEC Chromogen Kit

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Catalogue No: abx097196
Price: US$232.00
(Size: 1 Kit)
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Immunohistochemical staining of human liver carcinoma and human colon carcinoma formalin-fixed paraffin-embedded tissue, using AEC Chromogen Kit. The section was then counterstained with hematoxylin and mounted with neutral gum.
AEC Chromogen Kit

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Datasheet
AEC Chromogen Kit contains AEC (3-Amino-9-Ethylcarbazole), a widely used chromogen for immunohistochemical staining. AEC is a substrate for peroxidase and produces a red product that is soluble in alcohol and must be used with an aqueous counterstain and mounting media. It produces a clean, intense stain that is free of unwanted background.

Kit components:
  • PBS: 2 × 30 ml
  • Substrate Reagent: 3 ml
  • AEC Chromogen: 3 ml


Tested Applications WB, IHC, DB
Storage Store all components between 2-8 °C.
Availability Shipped within 7-12 working days.
Note This product is for research use only.
Directions for use
  1. Shortly before incubation, prepare the Working AEC Substrate Reagent by adding to a clean tube 1 ml PBS, 50 µl Substrate Reagent and 50 µl AEC Chromogen. Mix thoroughly.
  2. Apply 50-100 µl of Working AEC Substrate Reagent to each slide. Incubate for 5-10 minutes.
  3. When sufficient staining has been achieved, rinse slides in PBS for 3 minutes. Repeat this wash step for a total of 3 times.

Notes:
  • Do not use solutions containing alcohol for counterstaining, as the AEC stain is soluble in organic solvents.
  • The nitrocellulose strip will normally have a slight reddish background.
  • Use personal protective equipment (PPE) such as lab coats, gloves and goggles, when carrying out the assay.
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