Propidium Iodide (PI) Staining Kit

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Catalogue No: abx097208
Price: US$188.50
(Size: 1 Kit)

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Datasheet
Propidium Iodide (PI) Staining Kit contains propidium iodide (PI), a red fluorescent stain that binds to DNA by intercalating between the bases. PI is unable to enter living cells, and so it stains cells that have undergone apoptosis, necrosis or fixation.

Kit components:
  • Propidium Iodide Chromagen (1 mg/ml): 100 µl
  • Dilution Buffer: 20 ml


Target Propidium Iodide (PI)
Tested Applications IF/ICC
Recommended dilutions IF/ICC: 5 µg/ml (dye concentration). Optimal dilutions/concentrations should be determined by the end user.
Storage Store the Propidium Iodide Chromogen at -20 °C, and the dilution buffer at 4 °C. Avoid exposure to light.
Availability Shipped within 7-12 working days.
Note This product is for research use only.
Directions for use For double or triple fluorescence staining in immunofluorescence tests, PI staining is the last step after incubating the antibodies.

For cultured cells, add 10 µl of Propidium Iodide Chromogen to 2 ml Dilution Buffer in the same tube. Mix thoroughly (dye concentration is 5 μg/ml). Incubate for approximately 5 minutes in the dark at 30 °C, then wash with PBS/TBS 3 times, 3 minutes each time.
Research Articles on Propidium Iodide (PI)


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