Histone H3K9me3 Antibody

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Catalogue No: abx000007
Price: US$232.00
(Size: 20 µl)

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Datasheet SDS
Histone H3K9me3 Antibody is a Rabbit Polyclonal antibody against Trimethyl Histone H3K9. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Target Histone H3K9me3
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions WB: 1/100 - 1/500, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, DB: 1/500 - 1/2000, ChIP: 2.5 µg/5-10 µg Chromatin. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen A synthetic trimethylated peptide around K9 of human histone H3.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC Q16695 (UniProt, ExPASy)
UniProt Secondary AC B2R5K3, Q6FGU4
UniProt Entry Name H31T_HUMAN
Gene Symbol HIST3H3
GeneID 8290
NCBI Accession NP_003520.1
KEGG hsa:8290
String 9606.ENSP00000355657
Molecular Weight Calculated MW: 16 kDa
Observed MW: 17 kDa
Buffer PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration 0.472 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on Histone H3K9me3


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