Serine/threonine-Protein Kinase B-Raf (BRAF) Antibody

Catalogue No: abx000071
Price: £220.00
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BRAF Antibody is a Mouse Monoclonal antibody against BRAF. BRAF: v-raf murine sarcoma viral oncogene homolog B1, also known as BRAF1; RAFB1; B-RAF1; FLJ95109. Entrez Protein NP_004324. It is the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. B-Raf contains three consensus Akt phosphorylationsites (Ser364, Ser428, and Thr439). B-Raf is a key regulatory molecule of the mitogen-activated protein kinase kinase (MEK), it has a long amino-terminal region,the region is essential for homo-dimerization of B-Raf and hetero-dimerization of B-Raf and c-Raf at the plasma membrane, followed by phosphorylation of Thr118 in the amino-terminal B-Raf-specific region. Notably, in calcium ionophore-stimulated HeLa cells, B-Raf could propagate signals to MEK under the basal level of GTP-Ras. Expression of Raf-B is highly restricted with highestlevels in the cerebrum and testes and defects in braf are involved in a wide range of cancers. The BRAF gene mutation is frequently detected in papillary thyroid carcinoma,melanocytic nevi, primary cutaneous melanomas and colorectal cancers.

Target BRAF
Reactivity Human
Host Mouse
Clonality Monoclonal
Tested Applications WB
Recommended dilutions WB: 1/500 - 1/2000. Optimal dilutions/concentrations should be determined by the end user.
Immunogen Recombinant protein of human BRAF.
Purification Affinity purified.
Form Liquid
Isotype IgG
Conjugation Unconjugated
Storage Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight Calculated MW: 84 kDa
Observed MW: 105 kDa
Swiss Prot P15056
GeneID 673
Gene Symbol BRAF
Concentration > 1 mg/ml
Buffer PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
UNSPSC Code 12352203
Availability Shipped within 5-10 working days.
Note This product is for research use only.

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