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HDAC4/5/9 (pS246/259/220) Antibody is a Rabbit Polyclonal antibody against HDAC4/5/9 (pS246/259/220). Histone Deacetylases (HDACs) are a group of enzymes closely related to sirtuins. They catalyze the removal of acetyl groups from lysine residues in histones and non-histone proteins, resulting in transcriptional repression. In general,they do not act autonomously but as components of large multiprotein complexes, such as pRb-E2F and mSin3A, that mediate important transcription regulatory pathways. There are three classes of HDACs; classes 1, 2 and 4, which are closely related Zn2+-dependent enzymes. HDACs are ubiquitously expressed and they can exist in the nucleus or cytosol. Their subcellular localization is effected by protein-protein interactions (for example HDAC-14.3.3 complexes are retained in the cytosol) and by the class to which they belong (class 1 HDACs are predominantly nuclear whilst class 2 HDACs shuttle between the nucleus and cytosol). HDACs have a role in cell growth arrest, differentiation and death and this has led to substantial interest in HDAC inhibitors as possible antineoplastic agents.
||WB: 1/500 - 1/1000, IHC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
||Synthetic Peptide. A phospho specific peptide corresponding to residues surrounding S246/259/220 of human HDAC4/5/9.
||Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
||Calculated MW: 57-65 kDa/97-122 kDa
Observed MW: 125 kDa, 140 kDa
||HDAC4, HDAC5, HDAC9
||> 1 mg/ml
||PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
||Shipped within 5-10 working days.
||This product is for research use only.