Melan-A (MLANA) Antibody
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Price:
US$261.00
(Size: 40 µl)
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Melan-A (MLANA) Antibody is a Rabbit Monoclonal antibody for the detection of Melan A.
| Target |
Melan-A (MLANA) |
| Clonality |
Monoclonal |
| Clone |
A206 |
| Reactivity |
Human |
| Tested Applications |
IHC |
| Host |
Rabbit |
| Recommended dilutions |
IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation |
Unconjugated |
| Immunogen |
Synthetic peptide derived from the C-terminal region of human Melan A. |
| Isotype |
IgG |
| Form |
Liquid |
| Purification |
Purified from rabbit antiserum by proprietary techniques. |
| Storage |
Store at 2-8°C. |
| UniProt Primary AC |
Q16655 (UniProt,
ExPASy)
|
| Buffer |
20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3. |
| Availability |
Shipped within 5-10 working days. |
| Note |
This product is for research use only. |
| Directions for use |
Suggested IHC-P Protocol - Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
- Preparation of Wash Buffer: Use 0.05 M Tris-HCl, pH 7.6, containing 0.2% Tween-20.
- Deparaffinize the section in 3 changes of xylene, 5 minutes each.
- Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
- Rinse in distilled water.
- Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
- Wash in distilled water for 5 minutes.
- Antigen retrieval: immerse the slide in Citrate buffer, pH 6.0, and incubate in a water bath for 30-40 minutes at 96-98 °C.
- Remove the slide from the water bath and allow to stand at room temperature (in Citrate buffer, pH 6.0) for 15 minutes.
- Rinse in distilled water.
- Wash in Wash Buffer for 5 minutes.
- Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (> 1 mg/ml) to dilute this antibody.
- Wash twice with Wash Buffer, 5 minutes each.
- Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
- Wash twice in Wash Buffer, 5 minutes each.
- Apply the DAB chromagen for 1-3 minutes.
- Rinse in water for 10 minutes.
- Stain in hematoxylin for 5 minutes.
- Wash in water for 10 minutes.
- Dehydrate the section in 2 changes of 96% ethanol, 5 minutes each.
- Wash the section in 2 changes of xylene, 2 minutes each.
- Mount the slide for observation.
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