Polyclonal Antibody Production Service

Rabbit or Goat Polyclonal Antibody Production Service

Project 1 Protein Expression Route

Content:

1. Construction of highly expressed clone

2. Antigen preparation and purification

3. Animal immunization (2 Japanese white rabbits)

4. ELISA titer test

5. Antiserum

6. Antibody affinity purification

7. WB test (against the antigen)

Product: 100 μl preimmune serum/rabbit; 2mg affinity purified antibody/rabbit; Image of antigen purification; ELISA & WB test reports

Duration: 4 - 6 months


Project 2 Polypeptide Route

Content:

1. Polypeptide synthesis

2. KLH coupling

3. Animal immunization (2 Japanese white rabbits)

4. ELISA titer test

5. Antiserum

6. Antibody affinity purification

7. Dot-blot test (against naked polypeptide)

Product: 100 μl preimmune serum/rabbit; 2mg affinity purified antibody/rabbit; Atlas of polypeptide MS & HPLC test; Dot-blot test reports

Duration: 3 - 5 months


Project 3 Phosphorylated Antibodies

Content:

1. Synthesis modified & non-modified polypeptide

2. KLH coupling

3. Animal immunization (2 Japanese white rabbits)

4. ELISA titer test

5. Antiserum

6. Antibody affinity purification

7. Dot-blot test (against modified & non-modified polypeptide)

Product: 100 μl preimmune serum/rabbit; 2mg affinity purified antibody/rabbit; Atlas of polypeptide MS & HPLC test; Dot-blot test reports

Duration: 3 - 5 months


Project 4 Methylated & acetylated antibodies

Content:

1. Synthesis modified & non-modified polypeptide

2. KLH coupling

3. Animal immunization (2 Japanese white rabbits)

4. ELISA titer test

5. Antiserum

6. Antibody affinity purification

7. Dot-blot test (against modified & non-modified polypeptide)

Product: 100 μl preimmune serum/rabbit; 2mg affinity purified antibody/rabbit; Atlas of polypeptide MS & HPLC test; Dot-blot test reports

Duration: 3 - 5 months