Preloading Fluorescent Staining Solution

Preloading Fluorescent Staining solution is a non-mutagenic fluorescent reagent producing an instant visualization of DNA bands upon illuminating agarose gels on the UV Transilluminator. It is supplied in the 6X DNA Loading Buffer, is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process and for detecting the double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), and RNA at as low as 0.14 ng DNA, thus rendering it the most sensitive stain available on the market.

The recommended DNA sample mass is at least 50 ng or more, thus not causing any obvious shift in the migration pattern. Regardless of the loaded DNA or RNA sample amount for staining, it achieves cogently consistent migration pattern without the “smiley signature” commonly identified with other competing stains on the market. It is the ideal non-hazardous alternative to Ethidium Bromide for protecting the environment and meeting with the local biosafety regulations/ESG practices/UN SDG Goals.

Target	Preloading Fluorescent Staining Solution
Tested Applications	SDS-PAGE
Storage	Store at 4 °C for up to 12 months. For long-term storage, store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer	Contains < 0.01% DMSO, > 50% Glycerol.
Availability	Shipped within 10-15 working days.
Note	This product is for research use only.
Directions for use	Mix the staining solution before use. Dilute staining solution in a 1:5 ratio with DNA sample and DNA marker. Mix the solution gently. Load DNA samples and DNa marker and after after electrophoresis, remove the gel and place it on a UV-transilluminator to immediately visualize the bands. If required, post-stain with Ethidium bromide.

Research Articles on Preloading Fluorescent Staining Solution

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