1,25-Dihydroxyvitamin D3 (DHVD3) Antibody Pair for use in Sandwich ELISA assay development. The 10 × 96 tests size contains:
- 400 μg 1,25-Dihydroxyvitamin D3 (DHVD3) rabbit polyclonal capture antibody,
- 100 μg 1,25-Dihydroxyvitamin D3 (DHVD3) Biotinlylated Competitor,
- 1 μg 1,25-Dihydroxyvitamin D3 (DHVD3) standard.
| Target |
1,25-Dihydroxyvitamin D3 (DHVD3) |
| Reactivity |
Human |
| Tested Applications |
ELISA |
| Recommended dilutions |
Dilute the Capture Antibody 125-fold with Coating Buffer.
Dilute the Biotinylated Competitor 200-fold with Detection Antibody Diluent. Optimal dilutions/concentrations should be determined by the end user. |
| Form |
Liquid (Capture Antibody and Detection Antibody) |
| Reconstitution |
Reconstitute the standard with Standard Diluent. The volume, and therefore standard concentration, should be determined by the end user. |
| Test Range |
123.5 pg/ml - 10000 pg/ml |
| Storage |
Store at 2 to 8 °C for up to one month. Aliquot and store at -20 °C for up to one year. Avoid repeated freeze/thaw cycles. All solutions should be made fresh before the experiment. |
| Concentration |
400 μg / 0.8ml (Capture Antibody), 100 μg / 0.5 ml (Biotinylated Competitor), 10 μg/vial (Standard) |
| Standard Form |
Lyophilized |
| ELISA Type |
Sandwich |
| Capture Antibody Host |
Rabbit |
| Capture Antibody Clonality |
Polyclonal |
| Capture Antibody Conjugation |
Unconjugated |
| Detection Antibody Conjugation |
Biotin |
| Buffer |
The capture and detection antibody both contain 0.1% sodium azide. |
| Availability |
Shipped within 5-15 working days. |
| Note |
This product is for research use only. |
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