5′-Nucleotidase Assay Kit

Catalogue No: abx098400
Price: £400.00
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5′-Nucleotidase Assay Kit is an Assay Kit by Enzymatic Colorimetric Method.

Based on the bottle type, product model can be classified into Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott,Universal types, etc.

This product is used to determine 5′-Nucleotidase activity in human serum.

The kit is based on continuous monitoring assay use the 5′-Nucleotidase coupling purine nucleoside phosphorylase (PNP), xanthine oxidase (XOD) and peroxidase (POD). 5'-nucleotidase hydrolyze inosine acid to generate inosine, then coupled by the action of PNP to generate hypoxanthine. Uric Acid and hydrogen peroxide formed from hypoxanthine under Oxidation of XOD. Finally, Fuchsia colored quinone was produced under POD effect by the Trinder reaction. The 5'-nucleotidase activity can be calculated by measuring the increase rate of colored quinone absorbance at 550nm wavelength.

R1: Detection Reagent 1 R2: Detection Reagent 2
Glycine Buffer → 6g/L 5′-hypoxanthine nucleotide → 3.482g /L
Purine nucleoside phosphorylase → > 0.5KU/L 4-Aminoantipyrene → 0.406g /L
Xanthine oxidase → >0.8KU/LL
Peroxidase → >0.6KU/L
TOPS → 0.5g/L

This assay kit is suitable for automatic or semi-automatic biochemical analyzer with 546nm and 800nm wavelength.

Serum without hemolysis used as sample. Samples testing should be completed at the same day after collected. Otherwise, the samples should be cryopreserved and avoid repeated freeze-thaw cycles. 5′-Nucleotidase in the samples remains stable for 7days at 2-8°C and for 3 months at cryopreservation condition.

Target 5′-Nucleotidase
Form Liquid
Test Range 5 U/L - 100 U/L
Storage Stored for up to 12 months at 2-8°C, protect from light. After opening, the reagent remains stable for 28 days at 2-8°C, protect from light.
Availability Shipped within 5-12 working days.
Note This product is for research use only.

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