a-amylase Assay Kit is an Assay Kit by Enzymatic Method.
Based on the bottle type, product model can be classified into Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott, Universal types, etc.
This product is used to determine α-amylase activity in human serum.
P-nitrophenyl-alpha-D-maltoside is used as substrate which can be hydrolyzed to Et-Gx and Gy-pNP by AMY. α-Glucosidase hydrolyze Gy-pNP to generate glucose and pNP- glucoside. Final result of the two reactions is produced freedom p-NP with detected characteristic absorption peak at 405nm. The AMY activity in the sample is proportional to the level of p-NP.
|R1: Reagent Composition ||R2: Reagent Composition |
|Good’s buffer → 50mmol/L ||Ethidene-G7-pNP → 3.12g/L |
|CaCl2 → 1mmol/L || |
|a- Glucosidase → ＞3KU/L || |