a-amylase Assay Kit

Catalogue No: abx098401
Price: £170.00
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a-amylase Assay Kit is an Assay Kit by Enzymatic Method.

Based on the bottle type, product model can be classified into Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott, Universal types, etc.

This product is used to determine α-amylase activity in human serum.

P-nitrophenyl-alpha-D-maltoside is used as substrate which can be hydrolyzed to Et-Gx and Gy-pNP by AMY. α-Glucosidase hydrolyze Gy-pNP to generate glucose and pNP- glucoside. Final result of the two reactions is produced freedom p-NP with detected characteristic absorption peak at 405nm. The AMY activity in the sample is proportional to the level of p-NP.

R1: Reagent Composition R2: Reagent Composition
Good’s buffer → 50mmol/L Ethidene-G7-pNP → 3.12g/L
CaCl2 → 1mmol/L
a- Glucosidase → >3KU/L

This assay kit is suitable for automatic or semi-automatic biochemical analyzer with 405nm and 700nm wavelength.

The sample should be human serum (without hemolysis). Samples testing should be completed at the same day after collected.

Target a-amylase
Form Liquid
Storage Stored for up to 12 months at 2-8°C, protect from light. After opening, the reagent remains stable for 30 days at 2-8°C, protect from light.
Availability Shipped within 5-12 working days.
Note This product is for research use only.

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