α - Hydroxybutyrate Dehydrogenase Assay Kit is an Assay Kit by Latex Enhanced Method.
Based on the bottle type, product model can be classified in to Hitachi 7170, Hitachi 7060, Hitachi 7020, Beckman, Toshiba, Mindray, DuPont, Innova, Siemens, KHB, Abbott, Universal types, etc.
This reagent is used to determine the α - Hydroxybutyrate Dehydrogenase activity in human serum.
Under the catalysis of α - Hydroxybutyrate Dehydrogenase , α - Ketobutyric acid is reduced to α - hydroxybutyric acid, and NADH is oxidized to NAD +. At 340nm, the decrease rate of absorbance is directly proportional to α - hydroxybut yrate dehydrogenase activity , and the α - hydroxybutyrate dehydrogenase activity in the sample can be calculated by monitoring the absorbance decreasing rate.
|R1: Detection Reagent 1||R2: Detection Reagent 2|
|Tris Buffer → 12.1 g/l||NADH → Appropriate|
|α - ketobutyric acid → 0.306 g/L|