Annexin V (APC)

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Catalogue No: abx090615
Price: US$623.50
(Size: 100 tests)

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Datasheet
Annexin V is used as a probe to detect cells that have expressed phosphatidylserine (PS) on the cell surface, an event found in apoptosis as well as other forms of cell death. The Annexin V affinity assay typically uses a conjugate of annexin V and a fluorescent or enzymatic label, biotin or other tags, or a radioelement, in a suitable buffer (Annexin V binding to PS is Ca2+ dependent). The assay combines Annexin V staining of PS membrane events with the staining of the cell nucleus with PI or AAD-7 to distinguish living cells from dead cells. Annexin V apoptosis detection is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner (cytoplasmic-facing) leaflet of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. Detection can be analyzed by flow cytometry or by fluorescence microscopy.

This product contains Annexin V (APC) only and does not include 10X Binding Buffer, PI or 7-AAD reagents.

Target Annexin V
Reactivity General (All species)
Tested Applications FCM
Conjugation APC
Excitation/Emission 651/660
Laser Line 647
Form Liquid
Storage Store undiluted at 4 °C. Avoid exposure to light. Do not freeze.
Buffer PBS, pH 7.2, 0.09% NaN3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Directions for use Staining Protocol
  1. Dilute the 10X Binding Buffer solution to 1X Working Binding Buffer solution with distilled water.
  2. Harvest cells (about 1 × 105 cells per test), then wash once with cold PBS. Remove the PBS from the cell pellet.
  3. Wash again with cold 1X Working Binding Buffer, then centrifuge at 300 × g for 10 min at room temperature. Remove the Binding Buffer from the cell pellet.
  4. Resuspend cells in cold 1X Working Binding Buffer to a concentration of 1 × 106 cells/ml.
  5. Add 100 µl of cells (1 × 105 cells) to each appropriate tube.
  6. Add 5 µl of Annexin V-APC to the appropriate tubes.
  7. Gently vortex each tube and incubate for 10 minutes at room temperature in dark.
  8. Add 5 µl of PI or 7-AAD solution and incubate for 5 min at room temperature in dark.
  9. Wash cells once in PBS, then resuspend in PBS.
  10. Analyze by flow cytometry within 4 hours.
Research Articles on Annexin V


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