DNase (RNase-free)

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Catalogue No: abx098138
Price: US$768.50
(Size: 1500 U)
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DNase (RNase-free)

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Datasheet
Deoxyribonuclease I (DNase I) digests single and double-stranded DNA to oligodeoxyribonucleotides containing a 5' phosphate. DNase I activated by Mg2+ cleaves double-stranded DNA randomly at any site, while DNase I activated by Mn2+ cleaves double-stranded DNA at approximately the same site to form sticky-ends with 1-2 nucleotide overhangs or blunt-ends.

Kit contents:
  • DNase I (3 U/µl): 1500 U
  • 10X DNase I Reaction Buffer: 2 × 1 ml
  • 200 mM EDTA: 1 ml


Target DNase
Purity Does not contain other DNA endonucleases and exonucleases. Does not contain RNase.
Storage Store at -20°C. Avoid repeated freeze/thaw cycles.
Buffer Storage Buffer: 50 mM Tris-acetate (pH 7.5), 10 mM CaCl2, 50% (v/v) glycerol.
10X Reaction Buffer: 100 mM Tris-HCl (pH 7.5 @ 25 °C), 100 mM MgCl2, 1 mM CaCl2.
Biological Activity One unit (U) is defined as the amount of enzyme required for completely digesting 1 µg pBR322 DNA in 10 minutes at 37 °C.

Activity test conditions: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 µg pBR322 DNA.
Specificity 32 kDa (monomer)
Concentration 3 U/µl
Availability Shipped within 10-20 working days.
Note This product is for research use only. This product is shipped with dry ice.
Directions for use Assay Procedure:

  1. Add the following components to an RNase-free microcentrifuge tube:
    Component Volume
    RNA as required
    10X DNase I Reaction Buffer 1 µl
    DNase I 1 U/µg RNA
    RNase-Free WaterUp to 10 µl
  2. Incbate at 37 °C for 30 min.
  3. Terminate the reaction by adding 0.5 µl of 200 mM EDTA solution.
  4. Incubate at 65 °C for 10 min to inactivate DNase I.
  5. The treated RNA sample is ready for reverse transcription.

Notes:

  • Use 1 U of DNase I per µg of RNA. If the amount of RNA is less than 1 µg, use 1 U of DNase I.
  • At least 1 mM EDTA is required for each 1 mM Mg2+ to terminate the reaction. In the reaction system, the working concentration of Mg2+ is 10 mM, so 0.5 µl of 200 mM EDTA is required to terminate the reaction. The final concentration of EDTA is 10 mM.
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