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Agarose High EEO
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Catalogue No: abx299903
Price:
US$319.00
(Size: 100 g)
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DatasheetThe High EEO agarose is an agarose designed for the analysis of molecules that exhibit high migration to the cathode. Suitable for the analysis of different nucleic acid fragmnents, in separation techniques such as electrophoresis counterimmunoelectrophoresis, chromotography, blotting and others used in the field of biochemestry and molecular biology.
Specification:
| Moisture | ≤ 10% |
| Gel strength (1.5% gel) | ≥ 1200 g/cm2 |
| Gelling point (1.5% gel) | 41-45 °C |
| Melting point (1.5% gel) | 84-88 °C |
| Target | Agarose High EEO |
| Storage | Store at room temperature. |
| Availability | Shipped within 10-15 working days. |
| Note | This product is for research use only. |
| Directions for use | Preparation of agarose gel in the microwave. 1. Define the quantity needed to prepare the gel at the desired concentration. 2. Add necessary volume of run buffer (TAE, TBE to 1X). 3. Homogenize and microwave for 2 - 4 minutes, considering cycles of 30 seconds, until the sample is completely dissolved. Caution: Use necessary protection for the handling of hot material. Suggestion: Work in microwave at medium power. 4. Add selected staining agent (e.g. Ethidium Bromide or other) and homogenize 5. Allow to cool to 60° C - at room temperature - load electrophoresis mold and let gel. 6. Remove mold from wells, mount electrophoresis chamber, load samples and run buffer - same buffer with which the gel was prepared - and start the electrophoretic run. Preparation agarose gel in heating plate. 1. Define the mass needed to prepare the gel at the desired concentration. 2. Add necessary volume of run buffer (TAE, TBE to 1X). 3. Homogenize and carry out a heating plate with stirring. Once the boiling has started, measure 20 minutes (use a lid or refrigerant system to avoid concentrating the sample). 4. Add selected staining agent (e.g. Ethidium Bromide or other) and homogenize. 5. Allow to cool to 60° C - at room temperature - load electrophoresis mold and let gel. 6. Remove mold from wells, mount electrophoresis chamber, load samples and run buffer - same buffer with which the gel was prepared - and start the electrophoretic run. |
