Total Bile Acid (TBA) Assay Kit is an assay kit for the detection and quantification of Total Bile Acid (TBA) concentrations in serum using a microplate reader. With S-NAD+ as hydrogen receptor, 3 alpha-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.

Target	Total Bile Acid (TBA)
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Validity	The validity for this kit is 12 months.
Test Range	2.05 µmol/L - 120 µmol/L
Sensitivity	2.05 µmol/L
Detection Method	Colorimetric
Assay Data	Quantitative
Sample Type	Serum.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Total Bile Acid (TBA)

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