TP53BP1 Antibody

Catalogue No: abx000560
Price: £260.00
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TP53BP1 Antibody

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TP53BP1 Antibody is a Rabbit Polyclonal antibody against TP53BP1. p53-binding protein 1 (53BP1) was originally identified as a p53 binding partner that could enhance the transcriptional activity of p53. 53BP1 consists of two BRCA1 carboxy terminal (BRCT) domains that allow for binding to p53 and a separate domain responsible for binding to phosphorylated histone H2A.X. 53BP1 rapidly translocates to nuclear foci following treatment of cells with ionizing radiation (IR) or radiomimetic agents that cause DNA double strand breaks (DSBs). Because of this localization to DSBs and homology to the yeast protein Rad9, a role for 53BP1 in DSB repair has been proposed. Recruitment of 53BP1 to sites of DNA damage has been demonstrated to be independent of ATM, NBS1, and DNA-PK and retention of 53BP1 at DNA breaks requires phosphorylated H2A.X. In cells lacking 53BP1, phosphorylation of ATM substrates is reduced, suggesting that 53BP1 is upstream of ATM. In response to IR, phosphorylation of 53BP1 at serines 6, 25, 29, and 784 by ATM has been demonstrated, but phosphorylation at these sites is not required for localization of 53BP1 to sites of DSBs. Phosphorylation of 53BP1 at Ser1618 has been reported to be enriched in human cells arrested in mitosis.

Target TP53BP1
Reactivity Human, Mouse, Rat
Origin Human
Host Rabbit
Clonality Polyclonal
Tested Applications WB, IHC, IF/ICC
Recommended dilutions WB: 1/500 - 1/2000, IHC: 1/50 - 1/100, IF/ICC: 1/20 - 1/50. Optimal dilutions/concentrations should be determined by the end user.
Immunogen A synthetic peptide of human TP53BP1.
Purification Affinity purified.
Isotype IgG
Conjugation Unconjugated
Storage Aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles.
Molecular Weight 450 kDa
Swiss Prot Q12888
GeneID 7158
Gene Symbol TP53BP1
Concentration > 1 mg/ml
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Availability Shipped within 5-10 working days.
Note This product is for research use only.

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