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| Target | Forkhead Box Protein O1 Phospho-Thr24/32 (FOXO1 pT24 / 32) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1/5000, WB: 1/500 - 1/3000, IHC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human FOXO1/3/4-pan around the phosphorylation site of threonine 24/32 (S-C-TP-W-P). |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q12778 (UniProt, ExPASy) O43524 (UniProt, ExPASy) P98177 (UniProt, ExPASy) |
| Sequence | PRPRSCTWPLPRP |
| Buffer | PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol. |
| Specificity | Detects endogenous levels of total FOXO1/3/4-pan protein only when phosphorylated at threonine 24/32. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |