+44 (0) 1223 755950
+1 832 327 7413
HIST1H3B3 (pS10) Antibody
REQUEST MORE INFO
Catalogue No: abx031865
Price:
US$326.25
(Size: 50 µl)
Orders to United States ship from United States
(
Click on the image to see the image legend
Add to Cart
Buy It Now
GET A QUOTE
Documents
Datasheet SDS
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Research Articles on HIST1H3B3 (pS10)
| Target | HIST1H3B3 (pS10) |
| Clonality | Polyclonal |
| Target Modification | Ser10 |
| Modification | Phosphorylation |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF/ICC, DB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/500, IHC-P: 1/100, IF/ICC: 1/25, DB: 1/500. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S10 of human HIST1H3B3. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at 2-8°C for up to 2 weeks, or at -20°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | P68431 (UniProt, ExPASy) |
| UniProt Entry Name | H31_HUMAN |
| NCBI Accession | NP_003520.1 NP_003521.2 NP_003522.1 NP_003523.1 NP_003524.1 NP_003525.1 NP_003526.1 NP_003527.1 NP_003528.1 NP_066298.1 |
| KEGG | hsa:8350 hsa:8351 hsa:8352 hsa:8353 hsa:8354 hsa:8355 hsa:8356 hsa:8357 hsa:8358 hsa:8968 |
| String | 9606.ENSP00000484841 |
| Molecular Weight | Calculated MW: 15.4 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |