Histone H3K27me3 Antibody
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Catalogue No: abx000010
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US$232.00
(Size: 20 µl)
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Datasheet SDS
Histone H3K27me3 Antibody is a Rabbit Polyclonal antibody against Histone H3K27me3. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Research Articles on Histone H3K27me3
| Target | Histone H3K27me3 |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC, IP, ChIP |
| Host | Rabbit |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/500 - 1/1000, IHC-P: 1/50 - 1/200, DB: 1/500 - 1/1000, IF/ICC: 1/50 - 1/200, IP: 0.5 µg - 4 µg antibody per 400 µg - 600 µg extracts of whole cells, ChIP: 5 µg antibody per 5 µg - 10 µg of Chromatin, ChIP-seq: 1/20 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Synthetic peptide corresponding to Histone H3K27me3. The exact sequence is proprietary. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q16695 (UniProt, ExPASy) |
| UniProt Secondary AC | B2R5K3, Q6FGU4 |
| UniProt Entry Name | H31T_HUMAN |
| Gene Symbol | HIST3H3 |
| GeneID | 8290 |
| NCBI Accession | NP_003520.1 |
| KEGG | hsa:8290 |
| String | 9606.ENSP00000355657 |
| Molecular Weight | Calculated MW: 16 kDa Observed MW: 17 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | > 0.2 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |