Mycoplasma Prevention Reagent

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Catalogue No: abx098886
Price: US$304.50
(Size: 1 ml)

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Mycoplasma Prevention Reagent

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Datasheet SDS
Mycoplasma Prevention Reagent are ready-to-use concentrated stock solutions composed of three mycoplasma antibiotics, including macrolide, fluoroquinolone and tetracycline antibiotics, which can effectively interfere with DNA replication and protein expression of mycoplasma. This product can effectively prevent mycoplasma contamination during cell culture process, and also has a good effect on the prevention of common gram-positive and gram-negative bacteria. In the daily cell culture, it can be used as the substitute of common penicillin and streptomycin antibiotics. It has the advantage of low active working concentration and low cytotoxicity.
  • Prevent mycoplasma contamination effectively.
  • Active at low working concentration and low cytotoxicity, suitable for a wide range of cell types.
  • An alternative with wide range of effects to the prevention of common gram-positive and gram-negative bacteria.


Target Mycoplasma Prevention Reagent
Recommended dilutions Cell Culture: 1/1000. Optimal dilutions/concentrations should be determined by the end user.
Form Liquid
Storage Store at -20°C. Avoid exposure to light. Avoid repeated/freeze thaw cycles.
Shelf Life Up to 18 months.
Buffer The exact formulation is proprietary.
Concentration 1000X
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Preparation of culture medium:
  1. Thaw and mix Mycoplasma Prevention Reagent and penicillin/streptomycin-free culture medium thoroughly.
  2. Dilute Mycoplasma Prevention Reagent to 1/1000 in the culture medium.

Procedure for use:
  • Replace the culture medium with Mycoplasma Prevention Reagent-added medium.
  • It is recommended to test regularly for mycoplasma contamination using a mycoplasma detection kit: abx098882, abx298017.

Notes:

  • Prior to use, ensure that the cap of the bottle is sealed. Thaw to room temperature before use. Mix gently and use 70% ethanol to wipe the bottle surface, and then place it on a clean bench.
  • It is recommended to add this product to the fresh culture medium.
  • To reduce the number of passages for rapidly proliferating cells, it is recommended to reduce cell density or reduce serum concentration in the medium.
  • Mycoplasma Prevention Reagent has been tested in a variety of cell lines, including HepG2, HEK-293T, HEK-293, HeLa, N2a, A549, MCF-7, CHO, K562, NIH/3T3, Sp2/0, BHK-21, VERO, U937, and SY5Y.
  • This product can be used on primary cells with low cytotoxicity. If the cells are very sensitive, it is recommended to reduce the concentration.
Research Articles on Mycoplasma Prevention Reagent


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