Protein A Resin

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Catalogue No: abx098111
Price: US$580.00
(Size: 5 ml)

Orders to United States ship from United States

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Protein A Resin

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Datasheet SDS
Protein A Resin is an affinity chromatography resin with high binding capacity for IgG. Protein A Resin is suitable for purification of monoclonal antibodies, polyclonal antibodies and immunology complexes, such as IP and Co-IP. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose.

Specifications:
  • Resin: Cross-linked 4% agarose
  • Ligand: Recombinant Protein A
  • Shape: Sphere
  • Pore Size: 90 µm (45-165)
  • Support Density: 6 mg Protein A/ml wet gel
  • Binding Capacity: 40-50 mg h-IgG/ml wet gel
  • Maximum Flow Rate (25 °C): 300 cm/h
  • Recommended Flow Rate: < 150 cm/h
  • Highest Resisistance of Atmospheric Pressure: 0.3 MPa
  • pH Stability: 3-10
Affinity of Protein A/G for IgG types:
SourceIgG SubtypeAffinity for Protein AAffinity for Protein G
HumanIgG1++++++++
HumanIgG2++++++++
HumanIgG3-++++
HumanIgG4++++++++
MouseIgG1+++++
MouseIgG2a++++++++
MouseIgG2b++++++
MouseIgG3+++++
RabbitIgG+++++++
GoatIgG-++
HorseIgG++++++
DogIgG+++
CowIgG++++++
PigIgG++++++
MonkeyIgG++++++++


Target Protein A Resin
Storage Store with 20% ethanol at 2-8°C.
Shelf Life 24 months.
Buffer Note: Buffers are not included with this product.

Equilibration Buffer: 20 mM PBS, 150 mM KCl, pH 7.0
Elution Buffer: 20 mM citric acid, pH 3.0-4.0; or 100 mM glycine, pH 3.0; or 20 mM sodium acetate, pH 3.0-4.0
Neutralisation Buffer: 1 M Tris-HCl, pH 9.0
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Preparing the Protein A purification column:
  1. Thoroughly resuspend the protein A resin to achieve a homogeneous suspension of the resin in 20% ethanol storage buffer.
  2. Immediately transfer the resin into a purification column. Ensure that the bottom of the column is plugged with a stopper. Close the value of the column and allow the resin to settle.
  3. Equilibrate the column with 5-10 bed volume of equilibration buffer.
Preparing samples:

To avoid blocking the column, samples should be centrifuged and filtered through a 0.45 µm filter before loading.

Loading samples and washing:

Load samples and wash with 5-10 bed volume of equilibration buffer, and collect the flow-through in a tube

Elute:

Elute antibodies with elution buffer. Collect the elution containing the target immunoglobulin and immediately neutralise to pH > 7.0 with neutralisation buffer. The elution conditions are closely related with binding strength and stability of antibody. When necessary, optimise the elution buffer.

Regeneration of Protein A resin:
  1. Either:
    • Wash the column/resin with 3-5 bed volume of 0.1 M citric acid, or 0.1 M citric acid with 20% ethanol, and then 5 bed volume of PBS (pH 7.0); or
    • 3-5 bed volume of 0.05 M NaOH with 1 M NaCl, or 6 M GuHCl, and then 5 bed volume of deionised water.
  2. Store the column/resin in 20% ethanol.
Research Articles on Protein A Resin


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