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ABL1 (pY245) Antibody
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Catalogue No: abx031821
Price:
US$326.25
(Size: 80 µl)
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ABL1 is a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t (9;22) translocation results in the head-to-tail fusion of the BCR and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.
Research Articles on ABL1 (pY245)
| Target | ABL1 (pY245) |
| Clonality | Polyclonal |
| Target Modification | Tyr245 |
| Modification | Phosphorylation |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100, IF/ICC: 1/10 - 1/50. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y245 of human ABL1. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by protein G affinity chromatography. Then, the antibody fraction was peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody was eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P00519 (UniProt, ExPASy) |
| UniProt Secondary AC | A3KFJ3, Q13869, Q13870, Q16133, Q17R61, Q45F09 |
| UniProt Entry Name | ABL1_HUMAN |
| Gene Symbol | ABL1 |
| GeneID | 25 |
| OMIM | 189980 |
| HGNC | 76 |
| KEGG | hsa:25 |
| Ensembl | ENSG00000097007 |
| String | 9606.ENSP00000361423 |
| Molecular Weight | Calculated MW: 123 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |
