Type-2 Restriction Enzyme DpnI (DMT) Enzyme

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Catalogue No: abx098137
Price: US$754.00
(Size: 200 U)

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Datasheet SDS
DMT cuts the sequence GAmTC (where A is methylated) but does not cut the sequence GATC (where A is not methylated). This enzyme can cut DNA prepared from the most commonly used E. coli strains (dam+ strain), but does not cut PCR products. This product is suitable for use in in vitro site-directed DNA mutagenesis, and degradation of methylated plasmid templates.

Restriction site:
    CH3
    |↓
5' GA|TC 3'
3' CT|AG 5'
     ↑|
      CH3


Target Type-2 Restriction Enzyme DpnI (DMT)
Storage Store at -20°C. Stable for 12 months from date of receipt.
Biological Activity One unit (U) is defined as the amount of enzyme required for completely digesting 1 µg methylated pBR322 DNA at 37 °C (reaction time: 1 h, reaction volume: 50 µl).
Availability Shipped within 10-20 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.

This product is shipped with dry ice.
Research Articles on Type-2 Restriction Enzyme DpnI (DMT)


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