Blood Genomic DNA Kit (with Magnetic Stand)
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Price:
US$435.00
(Size: 50 rxns)
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Blood Genomic DNA Kit (with Magnetic Stand) is an ideal solution for isolating high-quality DNA by specific adsorption of magnetic beads, with no centrifugation required.
Kit components: - Binding Buffer: 18 ml
- Clean Buffer: 50 ml
- Wash Buffer: 12 ml
- Elution Buffer: 10 ml
- Proteinase K (20 mg/ml): 1 ml
- Magnetic Beads: 800 µl
- Magnetic Stand (16 hole): 1
| Target |
Blood Genomic DNA |
| Storage |
Store at room temperature (15-25°C). Stable for 12 months from date of receipt. |
| Sample Type |
Whole blood. |
| Material Required But Not Provided |
- Pipettes and pipette tips
- Microcentrifuge tubes
- Ethanol (100%): 98 ml
- Isopropanol (100%)
- Vortex
|
| Sample Collection/Preparation |
Whole blood: Use directly. Use within 1 week of collection if stored at 2-8 °C. For longer-term storage, store at -80 °C. Avoid repeated freeze-thaw cycles. |
| Reagent Preparation |
Bring all reagents to room temperature before use.
- Clean Buffer Working Solution: Add 50 ml of 100% Ethanol to 50 ml Clean Buffer and mix fully.
- Wash Buffer Working Solution: Add 48 ml of 100% Ethanol to 12 ml Wash Buffer and mix fully.
- Magnetic Beads: Mix well with a vortex immediately before use.
|
| Availability |
Shipped within 10-20 working days. |
| Note |
This product is for research use only. |
| Directions for use |
- Add 50-250 µl of whole blood to each 1.5 ml microcentrifuge tube.
- Add 300 µl of Binding Buffer and 20 µl of Proteinase K to the microcentrifuge tubes and mix well by vortexing.
- Stand at room temperature for 10 minutes, vortex 1-2 times during incubation.
- Add 450 µl Isopropanol to each microcentrifuge tubes and vortex for 10 seconds. Add 15 µl of well-mixed Magentic Beads into each microcentrifuge tubes.
- Vortex for 1 minute, then stand at room temperature for 3 minutes. Repeat for a total of four times.
- Place the microcentrifuge tubes on the magnetic stand. Gently turn the tubes left and right, then invert the stand 2-3 times. Stand at room temperature for 30 seconds. Remove as much supernatant as possible, being careful to not remove any beads.
- Remove the microcentrifuge tubes from the stand, add 800 µl of Clean Buffer Working Solution, vortex for 2 minutes, then place the tube back on the magnetic stand and remove the supernatant as described in step 6. Repeat for a total of two times.
- Remove the microcentrifuge tubes from the stand, add 500 µl of Wash Buffer Working Solution, vortex for 2 minutes, then place the tube back on the magnetic stand and remove the supernatant as described in step 6. Repeat for a total of two times.
- Uncap the tubes, and allow to air-dry for 10-15 minutes.
- Remove the tubes from the stand, add 100-200 µl of Elution Buffer to each tube, and mix gently by pipetting up and down several times to resuspend the beads. Incubate at 56 °C for 10 minutes, mixing gently by pipetting up and down 1-2 times during incubation.
- Place the tubes onto the stand, and separate beads as described in step 6. Carefully transfer the supernatant to a clean tube, and store the purified DNA at -20 °C
Note: Sterile pipette tips must be used to avoid contamination with DNase. |
Research Articles on Blood Genomic DNA
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