Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254) Antibody

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Catalogue No: abx012405
Price: US$58.00
(Size: 10 µg)

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Western blot analysis of extracts from HeLa cells treated with TNF-alpha (10 ng/ml, 30mins), using HSP90B (phospho-Ser254) antibody. The lane on the right is blocked with the phosphopeptide.
Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254) Antibody Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254) Antibody Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254) Antibody

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Datasheet SDS
Rabbit polyclonal antibody against HSP90B protein. Immunogen region is Internal. Specificity is as follows for the reactive species: H:S254, M:S254, R:S254.

Target Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254)
Clonality Polyclonal
Target Modification Ser254
Modification Phosphorylation
Reactivity Human, Mouse, Rat
Tested Applications ELISA, WB, IHC, IF/ICC
Host Rabbit
Recommended dilutions ELISA: 1/40000, WB: 1/500 - 1/3000, IHC: 1/50 - 1/100, IF/ICC: 1/100 - 1/500. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen The antiserum was produced against synthesized phosphopeptide derived from Human HSP90B around the phosphorylation site of serine 254 (V-G-SP-D-E).
Isotype IgG
Form Liquid
Purification Purified from Rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P08238 (UniProt, ExPASy)
UniProt Secondary AC B2R5P0, Q5T9W7, Q9NQW0, Q9NTK6
UniProt Entry Name HS90B_HUMAN
KEGG hsa:3326
String 9606.ENSP00000360609
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% sodium azide, 50% glycerol.
Specificity Detects endogenous levels of HSP90B only when phosphorylated at serine 254.
Concentration 1 mg/ml
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Research Articles on Heat Shock Protein 90 kDa Beta Phospho-Ser254 (HSP90B pS254)


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