10-250 kDa Protein Marker (Stained)

10-250 kDa Protein Marker (Stained) is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verifying of Western transfer efficiency on PVDF, nylon and nitrocellulose membranes and approximate sizing of proteins. It is composed of prestained recombinant prokaryotic proteins. Prestained Protein Molecular Weight Marker is a three-color molecular weight standard, contains 9 prestained natural proteins with molecular weights from 10 kDa to 250 kDa. It has two orange reference bands - one at 70 kDa and one at 25 kDa.

Target	10-250 kDa Protein Marker (Stained)
Tested Applications	WB
Storage	Aliquot and store between 4°C for up to 3 months. Aliquot and store at -20°C for long-term storage. Avoid repeated freeze/thaw cycles.
Buffer	62.5 mM Tris-H3PO4, pH 7.5 at 25°C, containing 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	Bring to room temperature or heat at 37°C for 2-3 minutes to fully dissolve precipitates and mix gently to ensure a homogenous solution. Do not boil. Load the following volumes (thickness 0.75-1.0 mm): Mini SDS-polyacrylamide gel: 5 µl per well Large SDS-polyacrylamide gel: 10 µl per well

Research Articles on 10-250 kDa Protein Marker (Stained)

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