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Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha (PIP5K1A) Antibody
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Catalogue No: abx033836
Price:
US$326.25
(Size: 80 µl)
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Overexpression of phosphatidylinositol phosphate 5-kinase alpha (PIP5KIalpha), which synthesizes PIP2, suppresses apoptosis, whereas a kinase-deficient mutant does not. Protection by the wild-type PIP5KIalpha isaccompanied by decreases in the generation of activated caspases and of caspase 3-cleaved PARP. Protection is not mediated through PIP3 or Akt activation. An anti-apoptotic role for PIP (2) is substantiated by the finding that PIP5KIalpha is cleaved by caspase 3 during apoptosis, and cleavage inactivates PIP5KIalpha in vitro. Mutation of the P (4) position (D279A) of the PIP5KIalpha caspase 3 cleavage consensus prevents cleavage in vitro, and during apoptosis in vivo. Significantly, the caspase 3-resistant PIP5KIalpha mutant is more effective in suppressing apoptosis than the wild-type kinase. PIP2 is a direct regulator of apical and effector caspases in the death receptor and mitochondrial pathways, and PIP5KIalpha inactivation contributes to the progression of apoptosis.
Research Articles on Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha (PIP5K1A)
| Target | Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha (PIP5K1A) |
| Clonality | Polyclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 19-50 amino acids from the N-terminal region of human PIP5K1A. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | Q99755 (UniProt, ExPASy) |
| UniProt Secondary AC | A8K4Q0, B4DIN0, Q99754, Q99756 |
| UniProt Entry Name | PI51A_HUMAN |
| NCBI Accession | NP_001129108.1 NP_001129109.1 NP_001129110.1 NP_003548.1 |
| KEGG | hsa:8394 |
| String | 9606.ENSP00000357883 |
| Molecular Weight | Calculated MW: 62.6 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |