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Target | Human IgG H&L |
Clonality | Polyclonal |
Reactivity | Human |
Tested Applications | ELISA, WB, IHC |
Host | Goat |
Recommended dilutions | ELISA: 1/25000 - 1/100000, WB: 1/2000 - 1/10000, IHC-F: 1/1000 - 1/5000. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation | HRP |
Immunogen | Human IgG (whole molecule). |
Isotype | IgG |
Form | Lyophilized |
Purification | Purified from antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid-phase absorbtion. |
Reconstitution | Reconstitute in 1 ml of deionised H2O. |
Storage | Store lyophilized at 2°C to 8°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
UniProt Primary AC | P01834 (UniProt, ExPASy) P01857 (UniProt, ExPASy) P01859 (UniProt, ExPASy) P01860 (UniProt, ExPASy) P01861 (UniProt, ExPASy) P0CG04 (UniProt, ExPASy) |
Gene Symbol | IGHG1, IGHG2, IGHG3, IGHG4, IGKC, IGLV2-14 |
Buffer | Prior to lyophilization: PBS containing 1% BSA and 0.01% Gentamicin sulphate. |
Specificity | Specific for Human IgG. Assay by IEP resulted in single precipitin arc against anti-peroxidase, anti-goat serum, human IgG and human serum. |
Concentration | Prior to lyophilization: 2.0 mg/ml |
Availability | Shipped within 3-7 working days. |
Note | This product is for research use only. |