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Sonic Hedgehog Protein (SHH) Antibody
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Catalogue No: abx015984
Price:
US$427.75
(Size: 100 µl)
Orders to United States ship from United States
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This gene encodes a protein that is instrumental in patterning the early embryo.It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites.Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar.The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing.More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo.Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres.HPE is manifested by facial deformities.It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities.Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly.
Research Articles on Sonic Hedgehog Protein (SHH)
| Target | Sonic Hedgehog Protein (SHH) |
| Clonality | Monoclonal |
| Reactivity | Human, Mouse, Monkey |
| Tested Applications | ELISA, WB, IHC, FCM |
| Host | Mouse |
| Recommended dilutions | ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Purified recombinant fragment of human SHH (AA: 26-161) expressed in E. coli. |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified from ascites by Protein G chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| GeneID | 6469 20423 716553 |
| Molecular Weight | 49.6 kDa |
| Buffer | PBS, containing 0.05% sodium azide. |
| Concentration | 1 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |