Serine/Threonine-Protein Kinase 39 (SPAK) Antibody
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Catalogue No: abx033798
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US$326.25
(Size: 80 µl)
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Datasheet SDS
SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.
Research Articles on Serine/Threonine-Protein Kinase 39 (SPAK)
| Target | Serine/Threonine-Protein Kinase 39 (SPAK) |
| Clonality | Polyclonal |
| Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Host | Rabbit |
| Recommended dilutions | WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | KLH-conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at 2-8°C for up to 2 weeks, or at -20°C for long term storage. Avoid repeated freeze/thaw cycles. |
| Shelf Life | 12 months. |
| UniProt Primary AC | Q9UEW8 (UniProt, ExPASy) |
| UniProt Secondary AC | O14774, Q53S90, Q53SL7, Q53SS1, Q9UER4, X5D9C8 |
| UniProt Entry Name | STK39_HUMAN |
| NCBI Accession | NP_037365.2 |
| String | 9606.ENSP00000348278 |
| Molecular Weight | Calculated MW: 59.5 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Specificity | Predicted to react with Rat STK39. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |