Serine/Threonine-Protein Kinase 39 (SPAK) Antibody

SPAK is a serine/threonine kinase containing an N-terminal series of proline and alanine repeats (PAPA box), followed by a serine/threonine kinase catalytic domain, a nuclear localization signal, a consensus caspase cleavage recognition motif, and a C-terminal region. Northern blot analysis detects ubiquitous expression, most abundantly in brain and pancreas. SPAK can phosphorylate itself and an exogenous substrate in vitro. SPAK immunoprecipitates from transfected mammalian cells in a complex with another serine/threonine kinase that phosphorylates catalytically inactive SPAK. SPAK activates the p38 MAP kinase pathway in cotransfection assays. Full-length SPAK is expressed in the cytoplasm in transfected cells, while a mutant corresponding to caspase-cleaved STK39 localizes predominantly in the nucleus.

Target	Serine/Threonine-Protein Kinase 39 (SPAK)
Clonality	Polyclonal
Reactivity	Human, Mouse
Tested Applications	ELISA, WB, IHC
Host	Rabbit
Recommended dilutions	WB: 1/1000, IHC-P: 1/50 - 1/100. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 346-376 amino acids from the Central region of human SPAK.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	Q9UEW8 (UniProt, ExPASy)
UniProt Secondary AC	O14774, Q53S90, Q53SL7, Q53SS1, Q9UER4, X5D9C8
UniProt Entry Name	STK39_HUMAN
NCBI Accession	NP_037365.2
String	9606.ENSP00000348278
Molecular Weight	Calculated MW: 59.5 kDa
Buffer	PBS containing 0.09% sodium azide.
Specificity	Predicted to react with Rat STK39.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Serine/Threonine-Protein Kinase 39 (SPAK)

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