Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 (DMEM/F-12) Powder

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Catalogue No: abx295010
Price: US$72.50
(Size: 1 × 10 L)

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Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 (DMEM/F-12) Powder

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Datasheet SDS
Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 (DMEM/F-12) powder is a combination of the DMEM and F-12 media. It is widely used fo mammalian cell culture. It contains a high concentration of glucose along with various amino acids, vitamins and inorganic salts essential for cell growth, though does not contain proteins, lipids or growth factors.

The 1 × 10 L size is provided as 1 bottle containing lyophilized powder for 10 L of 1X DMEM/F-12. The 1 × 50 L size is provided as 1 bottle containing lyophilized powder for 50 L of 1X DMEM/F-12.

Target Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 (DMEM/F-12)
Form Lyophilized
Reconstitution Reconstitute in sterile water.
Storage Store at 2-8 °C for up to 3 years. Prepared media can be stored at 2-8 °C for up to 1 year. Avoid exposure to light.
Buffer pH 6.2-6.8, containing 3151 mg/L D-Glucose, 2.5 mM L-Glutamine, 8.1 mg/L Phenol Red, 0.5 mM Sodium Pyruvate. Does not contain HEPES, NaHCO3.
Endotoxin Level < 1 EU/ml
Concentration Prior to lyophilization: 12.04 g/L
Osmolality: 240-280 mOsm/kg
Turbidity: < 5.0 NTU
Loss on drying: < 5%
Availability Shipped within 5-7 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use This product should be used with serum (or equivalent) supplementation.
Use aseptic techniques to avoid contamination.

Preparation:

  1. Ensure the final solution is 90% sterile or ultrapure water.
  2. Add the water to a sterile container, weigh out the powder at a concentration of 12.0 g/L.
  3. Stir for 20 mins to dissolve the powder completely.
  4. When the powder has dissolved, add NaHCO3 at a concentration of 2.483 g/L to the container and stir for 5-10 mins until dissolved.
  5. Adjust the pH to 7.20-7.30 with 1 mol/L NaHCO3 or 1 mol HCl. Since filtration slightly increases the pH, the pH provided is slighly lower than the target value.
  6. Sterile water can be added to adjust the volume to 100%.
  7. Sterilize the final solution using a 0.2 µm pore size filter under positive pressure.
  8. Ensure the solution is only stored in glass, culture medium (PET) bottles, or single-use storage bags with an oxygen barrier.
Research Articles on Dulbecco's Modified Eagle Medium / Nutrient Mixture F-12 (DMEM/F-12)


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