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Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Antibody
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Catalogue No: abx005569
Price:
US$290.00
(Size: 100 µl)
Orders to United States ship from United States
Reviews: 
(1 Publications and Reviews)
(1 Publications and Reviews)
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GAPDH Antibody is a Mouse Monoclonal antibody against GAPDH. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It was widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocated to the nucleus post modification such as S-nitrosylation. This antibody is a mouse monoclonal antibody raised against full length GAPDH of human origin. It can recognize the 36kda GAPDH protein in most cells/tissues. Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
Research Articles on Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
| Target | Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) |
| Clonality | Monoclonal |
| Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF/ICC |
| Host | Mouse |
| Recommended dilutions | ELISA: 1 µg/ml, WB: 1/5000 - 1/50000, IHC-P: 1/50 - 1/200, IF/ICC: 1/50 - 1/200. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH. |
| Isotype | IgG |
| Form | Liquid |
| Purification | Purified by affinity chromatography. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P04406 (UniProt, ExPASy) |
| UniProt Secondary AC | E7EUT4, P00354, Q53X65 |
| UniProt Entry Name | G3P_HUMAN |
| GeneID | 2597 |
| KEGG | hsa:2597 |
| String | 9606.ENSP00000229239 |
| Molecular Weight | Calculated MW: 36 kDa Observed MW: 36 kDa |
| Buffer | PBS, pH 7.3, containing 0.05% Proclin-300, 50% glycerol. |
| Concentration | 2.91 mg/ml |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |