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Human Epidermal Growth Factor Receptor (EGFR) Protein (Active)
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Catalogue No: abx600059
Price:
US$362.50
(Size: 20 µg)
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Human Epidermal Growth Factor Receptor (EGFR) Protein is a recombinant Human protein produced in Mammalian cells. It consists of amino acids 25-645, fused to an N-terminal 10 His-tag and a C-terminal Myc-tag.
Research Articles on Epidermal Growth Factor Receptor (EGFR)
| Target | Epidermal Growth Factor Receptor (EGFR) |
| Research Area | Cancer |
| Origin | Human |
| Expression | Recombinant |
| Host | Mammalian cells |
| Conjugation | Unconjugated |
| Form | Lyophilized |
| Purity | > 97% (SDS-PAGE) |
| Purification | Purified by affinity chromatography using IMAC. 0.2 µm filtered prior to lyophilization. |
| Reconstitution | Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/ml. It is recommended to add a final glycerol concentration of 5-50%. |
| Storage | Aliquot and store at 2-8°C for up to 1 week. Store at -20°C or -80°C for up to 1 year. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P00533 (UniProt, ExPASy) |
| UniProt Entry Name | EGFR_HUMAN |
| Gene Symbol | EGFR |
| KEGG | hsa:1956 |
| String | 9606.ENSP00000275493 |
| Molecular Weight | Calculated MW: 73.6 kDa Observed MW (SDS-PAGE): 110 kDa |
| Sequence Fragment | 25-645 AA. |
| Sequence | LEEKKV CQGTSNKLTQ LGTFEDHFLS LQRMFNNCEV VLGNLEITYV QRNYDLSFLK TIQEVAGYVL IALNTVERIP LENLQIIRGN MYYENSYALA VLSNYDANKT GLKELPMRNL QEILHGAVRF SNNPALCNVE SIQWRDIVSS DFLSNMSMDF QNHLGSCQKC DPSCPNGSCW GAGEENCQKL TKIICAQQCS GRCRGKSPSD CCHNQCAAGC TGPRESDCLV CRKFRDEATC KDTCPPLMLY NPTTYQMDVN PEGKYSFGAT CVKKCPRNYV VTDHGSCVRA CGADSYEMEE DGVRKCKKCE GPCRKVCNGI GIGEFKDSLS INATNIKHFK NCTSISGDLH ILPVAFRGDS FTHTPPLDPQ ELDILKTVKE ITGFLLIQAW PENRTDLHAF ENLEIIRGRT KQHGQFSLAV VSLNITSLGL RSLKEISDGD VIISGNKNLC YANTINWKKL FGTSGQKTKI ISNRGENSCK ATGQVCHALC SPEGCWGPEP RDCVSCRNVS RGRECVDKCN LLEGEPREFV ENSECIQCHP ECLPQAMNIT CTGRGPDNCI QCAHYIDGPH CVKTCPAGVM GENNTLVWKY ADAGHVCHLC HPNCTYGCTG PGLEGCPTNG PKIPS |
| Tag | N-terminal His tag and C-terminal Myc tag |
| Buffer | Prior to lyophilization: PBS, pH 7.4 containing 6% Trehalose. Volume prior to lyophilization: 27 μl per vial. |
| Activity | Active |
| Biological Activity | Biological activity was measured by binding ability in functional ELISA of EGFR protein (0.1 µg/well) with a recombinant EGFR antibody (0.007629 ng/ml - 1000 ng/ml). Detection antibody: abx341004 - Goat anti-Human IgG Fc gamma fragment specific antibody (1/10000 dilution). EC50: 0.6154 ng/ml - 0.7361 ng/ml. |
| Endotoxin Level | < 1.0 EU/µg as determined by LAL method. |
| Availability | Shipped within 5-15 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |