Mycoplasma Elimination Reagent (Myco-3)
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Price:
US$275.50
(Size: 10 ml)
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Mycoplasma Elimination Reagent (Myco-3) belongs to a group of fluoroquinolone antibiotics. Most of the mycoplasma species in contaminated cells are sensitive to Myco-3. It works by effectively interfering with DNA replication of mycoplasma. It can eliminate mycoplasma contamination with minimal cytotoxicity and is suitable for a wide range of cell types. It is a ready-to-use product and is active at a low working concentration.
| Target |
Mycoplasma Elimination Reagent (Myco-3) |
| Form |
Liquid |
| Storage |
Store at -20 °C in the dark. |
| Buffer |
The exact formulation is proprietary. |
| Availability |
Shipped within 10-20 working days. |
| Note |
This product is for research use only. |
| Directions for use |
Procedure: - Preparation of the culture medium: Thaw and mix penicillin-free and streptomycin-free culture medium thoroughly. To 99 ml of this culture medium, add 1 ml of Mycoplasma Elimination Reagent (Myco-3).
- When performing cell passage or continuing to culture with mycoplasma contaminated cells, replace the culture medium with Myco-3-added medium and maintain the contaminated cells in the medium for 43 days.
- Continue the treatment for a total of 10-14 days by changing with the medium (containing Myco-3) every 2-3 days. For most mycoplasma contamination, it may be necessary to maintain the cells in the medium for 10-14 days until the mycoplasma is removed.
- During the process, the cells can be tested for mycoplasma contamination to detect the removal efficiency. In general, mycoplasma will be removed after 2 weeks of treatment. If the culture still tests positive, continue the treatment for another 3-7 days.
Notes: - Prior to use, ensure that the cap of the bottle is sealed. Thaw to room temperature before use. Mix gently and use 70% ethanol to wipe the bottle surface, and then place it on the clean bench.
- It is recommended to add this product to the fresh culture medium.
- For the fast proliferation of cell lines, in order to reduce the number of passage, it is recommended to reduce the density of cells or reduce the concentration of serum in medium.
- It is recommended to reduce the concentration of Myco-3, if the cells are very sensitive to Myco-3.
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