Mouse IgG1 Isotype Control

Catalogue No: abx139001
Price: £190.00
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Mouse IgG1 Isotype Control is an Isotype Control.

Target Mouse IgG1
Host Mouse
Clonality Monoclonal
Tested Applications WB, IHC, FCM, IP
Recommended dilutions Optimal dilutions/concentrations should be determined by the end user.
Purity > 95% (SDS-PAGE)
Purification Purified from cell culture supernatant by protein-G affinity chromatography.
Form Liquid
Isotype IgG1
Conjugation Unconjugated
Specificity This mouse IgG1 kappa monoclonal antibody has unknown specificity and was chosen as an isotype control after screening on variety of resting, activated, live and fixed rat and human tissues.
Storage Store at 2-8°C. Do not freeze.
Concentration 1 mg/ml
Buffer PBS with 15 mM sodium azide, approx. pH 7.4.
Directions for use This reagent is intended to be used as an isotype control for flow cytometry analysis to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this isotype control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg per ml of incubation mixture), change the conditions of your experiment to reduce the background.
Availability Shipped within 5-12 working days.
Note This product is for research use only.

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