MutL Homolog 1 (MLH1) Antibody

Catalogue No: abx000639
Price: £120.00
(Size: 20 µl)
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MLH1 Antibody is a Rabbit Polyclonal antibody against MLH1. Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). 1.Modrich, P. (2006) J Biol Chem 281, 30305-9. 2.Seng, T.J. et al. (2008) Br J Cancer 99, 375-82. 3.Harley, I. et al. (2008) Gynecol Oncol 109, 384-7. 4.Mao, G. et al. (2008) J Biol Chem 283, 3211-6. 5.Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1. 6.Vasen, H.F. (2005) Fam Cancer 4, 219-25. 7.Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Target MLH1
Clonality Polyclonal
Reactivity Human, Mouse, Rat
Tested Applications WB
Host Rabbit
Recommended dilutions WB: 1/500 - 1/2000, IF/ICC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Recombinant protein of human MLH1.
Isotype IgG
Form Liquid
Purification Purified by affinity chromatography.
Storage Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC P40692 (UniProt, ExPASy)
UniProt Secondary AC B4DI13, B4DQ11, E9PCU2
UniProt Entry Name MLH1_HUMAN
Gene Symbol MLH1
GeneID 4292
KEGG hsa:4292
String 9606.ENSP00000231790
Molecular Weight Calculated MW: 58 kDa, 73 kDa, 84 kDa
Observed MW: 94 kDa
Buffer PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Concentration > 0.5 mg/ml
Availability Shipped within 5-10 working days.
Note This product is for research use only.
Research Articles on MLH1

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