DNA Mismatch Repair Protein Mlh1 (MLH1) Antibody

MLH1 Antibody is a Rabbit Polyclonal antibody against MLH1. Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-α), PMS2 (MutL-β) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7). 1.Modrich, P. (2006) J Biol Chem 281, 30305-9. 2.Seng, T.J. et al. (2008) Br J Cancer 99, 375-82. 3.Harley, I. et al. (2008) Gynecol Oncol 109, 384-7. 4.Mao, G. et al. (2008) J Biol Chem 283, 3211-6. 5.Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1. 6.Vasen, H.F. (2005) Fam Cancer 4, 219-25. 7.Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Target	DNA Mismatch Repair Protein Mlh1 (MLH1)
Clonality	Polyclonal
Reactivity	Human, Mouse, Rat
Tested Applications	WB
Host	Rabbit
Recommended dilutions	WB: 1/500 - 1/2000, IF 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MLH1.
Isotype	IgG
Form	Liquid
Purification	Purified by affinity chromatography.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P40692 (UniProt, ExPASy)
UniProt Secondary AC	B4DI13, B4DQ11, E9PCU2
UniProt Entry Name	MLH1_HUMAN
Gene Symbol	MLH1
GeneID	4292
OMIM	114500
NCBI Accession	NP_000240.1 NM_000249.3
HGNC	7127
KEGG	hsa:4292
Ensembl	ENSG00000076242
String	9606.ENSP00000231790
Molecular Weight	Calculated MW: 85 kDa Observed MW: 85 kDa
Buffer	PBS, pH 7.3, containing 0.01% thimerosal, 50% glycerol.
Concentration	> 0.2 mg/ml
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on DNA Mismatch Repair Protein Mlh1 (MLH1)

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