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DNA Mismatch Repair Protein Msh2 (MSH2) Antibody
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Catalogue No: abx034997
Price:
US$326.25
(Size: 80 µl)
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Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Research Articles on DNA Mismatch Repair Protein Msh2 (MSH2)
| Target | DNA Mismatch Repair Protein Msh2 (MSH2) |
| Clonality | Monoclonal |
| Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Host | Mouse |
| Recommended dilutions | WB: 1/2000, IHC-P: 1/1000. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user. |
| Conjugation | Unconjugated |
| Immunogen | Purified His-tagged Human MSH2 protein |
| Isotype | IgG1 |
| Form | Liquid |
| Purification | Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
| UniProt Primary AC | P43246 (UniProt, ExPASy) |
| UniProt Secondary AC | B4E2Z2, O75488 |
| UniProt Entry Name | MSH2_HUMAN |
| Gene Symbol | MSH2 |
| GeneID | 4436 |
| OMIM | 114500 |
| NCBI Accession | NP_000242.1 NP_001245210.1 |
| HGNC | 7325 |
| KEGG | hsa:4436 |
| Ensembl | ENSG00000095002 |
| String | 9606.ENSP00000233146 |
| Molecular Weight | Calculated MW: 105 kDa |
| Buffer | PBS containing 0.09% sodium azide. |
| Availability | Shipped within 5-10 working days. |
| Note | THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION. |