Mucin 1 (MUC1) Antibody

REQUEST MORE INFO
Catalogue No: abx227088
Price: US$232.00
(Size: 40 µl)

Orders to United States ship from United States

Reviews: 0 Publications and Reviews
(0 Publications and Reviews)
IHC-P analysis showing Epithelial membrane antigen expression in sebaceous glands of the skin (4 µm section), stained with anti-CD227 monospecific antibody.
Mucin 1 (MUC1) Antibody Mucin 1 (MUC1) Antibody

Click on the image to see the image legend

Available Options

* Size:





Add to Shopping Cart Buy It Now GET A QUOTE

Documents

Datasheet SDS
Mucin 1 (MUC1) Antibody is a Rabbit Monoclonal antibody for the detection of EMA (CD227, Mucin-1).

Target Mucin 1 (MUC1)
Clonality Monoclonal
Clone V227
Reactivity Human
Tested Applications IHC
Host Rabbit
Recommended dilutions IHC-P: 1/100 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation Unconjugated
Immunogen Synthetic peptide derived from the C-terminal region of human Mucin-1.
Isotype IgG
Form Liquid
Purification Purified from rabbit antiserum by proprietary techniques.
Storage Store at 2-8°C.
UniProt Primary AC P15941 (UniProt, ExPASy)
UniProt Secondary AC A5YRV1, A6ZID9, A6ZIE0, B1AVQ8, B1AVR0, B6ECA1, E7ESE5, E7EUG9, P13931, P15942, P17626, Q0VAP5, Q0VAP6, Q14128, Q14876, Q16437, Q16442, Q16615, Q6S4Y3, Q7Z547, Q7Z548, Q7Z550, Q7Z552, Q9BXA4, Q9UE75, Q9UE76, Q9UQL1, Q9Y4J2
UniProt Entry Name MUC1_HUMAN
Gene Symbol MUC1
GeneID 4582
OMIM 113720
NCBI Accession NP_001018016.1 NM_001018016.2
HGNC 7508
KEGG hsa:4582
Ensembl ENSG00000185499
String 9606.ENSP00000484824
Buffer 20 mM Tris-HCl, pH 8.0, containing 20 mg/ml BSA and 0.05% NaN3.
Availability Shipped within 5-10 working days.
Note THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use Suggested IHC-P Protocol
  1. Preparation of Citrate Buffer (10 mM Citric Acid, 0.05% Tween-20, pH 6.0): Dissolve 1.92 g of anhydrous citric acid in 700 ml of distilled water. Adjust pH to 6.0 with 1 M NaOH and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  2. Preparation of Tris-EDTA Buffer (10 mM Tris Base, 1 mM EDTA solution, 0.05% Tween-20, pH 9.0): Mix 1.21 g Tris and 0.37 g EDTA and dissolve in 700 ml of distilled water. Adjust pH to 9.0 with 1 M HCl and then add 0.5 ml of Tween-20 and mix thoroughly. Adjust the final volume to 1 L with distilled water. Store this solution at room temperature for up to 3 months or at 4 °C for long-term storage.
  3. Preparation of Wash Buffer: Use PBS, pH 7.0-7.5, containing 0.05% Tween-20.
  4. Deparaffinize the section in 3 changes of xylene, 10 minutes each.
  5. Wash the section in 96%, 80% and 70% ethanol, 10 minutes each.
  6. Rinse twice in distilled water, 5 minutes each.
  7. Block endogenous peroxidase by incubating the tissue in 3% hydrogen peroxide (H2O2) for 10 minutes.
  8. Wash twice in distilled water, 5 minutes each.
  9. Antigen retrieval: immerse the slide in either Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 9.0), and incubate in a water bath for 20-25 minutes at 95-97 °C.
  10. Remove the slide from the water bath and allow to stand at room temperature for 15 minutes in the buffer used in the previous step.
  11. Rinse twice in distilled water, 5 minutes each.
  12. Wash twice in Wash Buffer, 5 minutes each.
  13. Incubate the section with primary antibody at 1/100 - 1/200 dilution for 1 hour in a closed wet chamber. It is recommended to use abx291502 Primary Antibody Diluent or a diluent containing protease-free BSA (≥ 1 mg/ml) to dilute this antibody.
  14. Wash 3 times with Wash Buffer, 5 minutes each.
  15. Add the secondary antibody and proceed to standard immunohistochemistry protocol (HRP - Peroxide - DAB). It is recommended to use abx291501 Rabbit and Mouse HRP/DAB Detection Kit.
  16. Wash 3 times with Wash Buffer, 5 minutes each.
  17. Apply the DAB chromagen for 1-3 minutes.
  18. Wash twice with distilled water, 5 minutes each.
  19. Stain in hematoxylin for 5 minutes.
  20. Wash 3 times with distilled water, 2 minutes each.
  21. Mount the slide for observation.
Research Articles on Mucin 1 (MUC1)


Write a review

Continue
Tags:
(Click to show)