Nestin (NES) Antibody

Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Target	Nestin (NES)
Clonality	Polyclonal
Reactivity	Human
Tested Applications	ELISA, WB, IHC, IF/ICC, FCM
Host	Rabbit
Recommended dilutions	WB: 1/2000, IHC-P: 1/25, IF/ICC: 1/25, FCM: 1/25. Not tested in IHC-F. Optimal dilutions/concentrations should be determined by the end user.
Conjugation	Unconjugated
Immunogen	KLH-conjugated synthetic peptide between 1389-1416 amino acids from human Nestin.
Isotype	IgG
Form	Liquid
Purification	Purified through a protein A column, followed by peptide affinity purification.
Storage	Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC	P48681 (UniProt, ExPASy)
UniProt Secondary AC	O00552, Q3LIF5, Q5SYZ6
UniProt Entry Name	NEST_HUMAN
NCBI Accession	NP_006608.1
KEGG	hsa:10763
String	9606.ENSP00000357206
Molecular Weight	Calculated MW: 177 kDa
Buffer	PBS containing 0.09% sodium azide.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.

Research Articles on Nestin (NES)

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