100 mM dNTP Set

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Catalogue No: abx299723
Price: US$348.00
(Size: 8 × 250 µl)
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PCR analysis of 100 mM dNTP set. Briefly, A 35 cycle PCR reaction is set up to test the amplification efficiency of 10mM dNTPs Mix. Bacteriophage Lambda DNA (1 ng/reaction) was used as PCR template. Primer sets of amplicons with 0.5, 1, 2, 3 kb were used in the reaction. After the PCR, 5 µl was analyzed with 1.5% agarose gel (TAE buffer). 0.1-3 kbp DNA marker (<a href="https://www.abbexa.com/index.php?route=product/search&search=abx299702" target="_blank">abx299702</a>) was run as molecular weight standard. All samples were stained with 1 µl DNA Staining Reagent (<a href="https://www.abbexa.com/index.php?route=product/search&search=abx299713" target="_blank">abx299713</a>).
100 mM dNTP Set

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Datasheet SDS
100 mM dNTP Set consists of all four deoxynucleotides (dATP, dCTP, dGTP, dTTP), two vials each at a concentration of 100 mM. Each vial is a ready-to-use solution and contains 250 µl of 25 µmol dNTP in purified water (pH 7.5). The deoxynucleotides are suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT tailing reactions.

Target 100 mM dNTP Set
Tested Applications RT-PCR
Form Liquid
Storage Store at -20 °C. Avoid repeated freeze/thaw cycles.
Buffer Purified distilled water, pH 7.5.
Concentration 100 mM
Availability Shipped within 10-15 working days.
Note This product is for research use only.
Directions for use 20 µl Final Reaction Volume:

Final dNTP Concentration dNTP Volume
0.2 mM0.04 µl
0.5 mM0.10 µl
1.0 mM0.20 µl
1.5 mM0.30 µl

25 µl Final Reaction Volume:

Final dNTP Concentration dNTP Volume
0.2 mM0.050 µl
0.5 mM0.125 µl
1.0 mM0.250 µl
1.5 mM0.375 µl

50 µl Final Reaction Volume:

Final dNTP Concentration dNTP Volume
0.2 mM0.10 µl
0.5 mM0.25 µl
1.0 mM0.50 µl
1.5 mM0.75 µl
Research Articles on 100 mM dNTP Set


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