14-3-3 Protein Sigma (SFN) Antibody is a Rabbit Polyclonal antibody for the detection of SFN.
14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms, denoted 14-3-3 b, g, e, z, h, q and s, comprise this family of signaling intermediates. 14-3-3 s, also known as SFN, stratifin, HME1 or YWHAS, is a secreted adaptor protein that is involved in regulating both general and specific signaling pathways. Expressed predominately in stratified squamous keratinising epithelium, 14-3-3 s is able to bind and modify the activity of a large number of proteins, such as KRT17 (Keratin 17), through recognition of a phosphothreonine or phosphoserine motif. When bound to Keratin 17, for example, 14-3-3 s acts to stimulate the Akt/mTOR signaling pathway by upregulating protein synthesis and cell growth. 14-3-3 s also functions to positively mediate IGF-I-induced cell cycle progression and can bind to a variety of translation initiation factors, thus controlling mitotic translation. In response to tumor growth, 14-3-3 s positively regulates the tumor suppressor p53 and increases the rate of p53-regulated inhibition of G2/M cell cycle progression. Multiple isoforms of 14-3-3 s exist due to alternative splicing events.
Target |
14-3-3 Protein Sigma (SFN) |
Clonality |
Polyclonal |
Reactivity |
Human, Mouse, Rat |
Tested Applications |
ELISA, WB, IHC, IF/ICC |
Host |
Rabbit |
Recommended dilutions |
WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/100. Optimal dilutions/concentrations should be determined by the end user. |
Conjugation |
Unconjugated |
Immunogen |
14-3-3 protein sigma |
Isotype |
IgG |
Form |
Liquid |
Purity |
≥ 95% (SDS-PAGE) |
Purification |
Purified by immunogen affinity chromatography. |
Storage |
Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles. |
Validity |
12 months. |
UniProt Primary AC |
P31947 (UniProt,
ExPASy)
|
UniProt Secondary AC |
Q6FH30, Q6FH51, Q96DH0 |
UniProt Entry Name |
1433S_HUMAN |
Gene Symbol |
SFN |
GeneID |
2810
|
OMIM |
601290
|
HGNC |
10773
|
KEGG |
hsa:2810
|
Ensembl |
ENSG00000175793 |
String |
9606.ENSP00000340989
|
Molecular Weight |
Observed MW: 31 kDa |
Buffer |
PBS, pH 7.3, with 0.02% sodium azide and 50% glycerol. |
Concentration |
2 mg/ml |
Availability |
Shipped within 5-12 working days. |
Note |
This product is for research use only. |
Research Articles on 14-3-3 Protein Sigma (SFN)