7-AAD Viability Staining Buffer

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with strong affinity for DNA. It can be used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry, and is useful for dead cell discrimination during flow cytometric analysis. It intercalates in double-stranded DNA, with high affinity for GC-rich regions, making it useful for chromosome banding studies. With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium-neon laser and can also be excited with lower efficiency using 488 nm or 514 nm argon laser lines.

Tested Applications	FCM
Recommended dilutions	FCM: 5 µl/test. Dilution is not necessary.
Form	Liquid
Storage	Store undiluted at 4°C. Avoid exposure to light. Do not freeze.
Shelf Life	12 months.
Buffer	PBS, pH 7.2, containing 0.09% NaN3.
Availability	Shipped within 5-10 working days.
Note	THIS PRODUCT IS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC, THERAPEUTIC OR COSMETIC PROCEDURES. NOT FOR HUMAN OR ANIMAL CONSUMPTION.
Directions for use	After adding the 7-AAD Viability Staining Buffer, incubate at room temperature for 5 minutes before analysis.

Research Articles on 7-AAD Viability Staining Buffer

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