7-AAD Viability Staining Buffer

7-Aminoactinomycin D (7-AAD) is a fluorescent chemical compound with strong affinity for DNA. It can be used as a fluorescent marker for DNA in fluorescence microscopy and flow cytometry. It is useful for dead cell discrimination during flow cytometric analysis. It intercalates in double-stranded DNA, with high affinity for GC-rich regions, making it useful for chromosome banding studies. With an absorption maximum at 546 nm, 7-AAD is efficiently excited using a 543 nm helium-neon laser; it can also be excited with somewhat lower efficiency using 488 nm or 514 nm argon laser lines.

Tested Applications	FCM
Recommended dilutions	FCM: 5 µl/test. Dilution is not necessary.
Form	Liquid
Storage	Store undiluted at 4 °C. Avoid exposure to light. Do not freeze.
Buffer	Contains PBS, pH 7.2, 0.09% NaN3.
Availability	Shipped within 5-10 working days.
Note	This product is for research use only.
Directions for use	After adding the 7-AAD Viability Staining Buffer, incubate at room temperature for 5 minutes before analysis.

Research Articles on 7-AAD Viability Staining Buffer

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